CELLCOVER


Protection, made by Anacyte Laboratories

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What is CellCover:

CellCover is a non-toxic formulation for protecting DNA, RNA and protein in human and animal solid tissues, including tumors, white blood cells and cultured cells (adherent, suspension, spheroids). It was developed for fast “one step” stabilizing of biomolecules in tissue specimens and cultured cells. For product specification Application Guides and Datasheets follow this link.

How it works:

As soon as cells get exposed to CellCover their metabolic state is frozen without applying low temperatures- liquid frozen. Synthesis and turnover of bio-molecules are stalled. This includes instant disruption of cellular degradation pathways. Chemical degradation is inhibited with CellCover as well, both leading to exceptional stabilization of DNA, RNA and protein. 


Time course: Cultured cells (SKMel-28) stored for 12 days in CellCover. RNA integrity was mesuared with Agilent Bioanalyzer. Results show a stable RIN value of 10 from first till last day of the time course.


The Benefits:

If treated with CellCover liquid freezing, cells grown on almost any substrate maintain in vivo morphology without chemical crosslinking of biomolecules. Compared to conventional formaldehyde fixation, formalin sensitive epitopes are readily accessible for antibodies in immunohistochemical experiments. A unique and special feature of CellCover liquid freezing is that you can perform additional molecular analysis on cells already analysed on morphological or molecular level: DNA, RNA (with high RINs) or protein can be isolated for subsequent applications after initial IHC, ICC or FACS analyses. Even integrity of high molecular weight RNA is protected (e.g precurser rRNA, which are normally degradated after applying commonly used standards procedures) - and there is no need to hurry!


CellCover fixed cell on glass slide. Positive IHC signal for formalin-sensitive Epitope Vimentin.

 


Primary cells shows high cellular integrity by FACS analysis.

 

Formalin fixed cell on glass slide. No IHC signal for formalin-sensitive Epitope Vimentin.

 


Gated cells (R1) with red and green fluorescence signals.

 


The opportunities: 

These unique properties now enable new types of analyses of cellular functions. With CellCover liquid freezing, subpopulations of cells can be separated according to known properties by flow cytometry and subsequently analysed for gene expression profiles on RNA or on protein-level. Thus, with CellCover liquid freezing it is easy to link transcriptome and proteome of a given cell population. In comparison to commonly used molecular biological methods, CellCover adds following new analytical tools to bio-molecular research:


Stabilize RNA and Protein simultaneously for several days, without doubt of molecule integrity


Reliable analysis HMW-RNA and low stability enzymes even after day of storage


Apply immunohistochemistry on formalin sensitive epitopes


Apply in situ hybridization for analysis of gene expression


Store cells conveniently for several days without change of protein or RNA expression profile or change in cells morphology as found with methanol


Isolate subpopulations by flow cytometry based on protein labelling and perform subsequent transcriptome and/or proteome (MALDI) analyses. 

RIN value 10. Precursor peak indicates protected high molecular weight RNA in CellCover stored cells.

 


Standard procedures of RNA isolation. High quality RNA after storing cells in CellCover.

 



Order information

You can place your orders with us directly either online using our Web-Shop, or email . Please note that the protocol included will be in the English language for every international destination.

Get more information about national shipping (Germany) or international shipping and if you have any further questions, please contact us.