Optimize Your Trypsinization Protocol by Use of CellCover.
As soon as cells experienced changes in their environment they adapt signal transduction and gene expression to it. Thus, upon temperature change and/or cell culture medium removal and trypsinization, cells adapt transcriptome and proteome very rapidly.
Keeping the true cell. The rapid fixation of RNA and proteins is very important for expression patterns to remain unchanged. Such rapid fixation is often at the expense of other factors such as molecule crosslinking by PFA or cell lysis by high-salt reagents that stabilize RNA. Not so with CellCover, which instantly fixes and stabilizes cells in their true state of RNA and protein expression.
Have you ever fixed your cells directly in their culture flask and then performed trypsinization for further experiments?
Probably not. But with CellCover you can, saving materials, time, and money.
Most importantly, with CellCover fixation followed by trypsinization, excellent sample quality is maintained with no time given for changes in the sample to occur.
Not only perfectly fixed RNA is retained after trypsinization, but typical cellular morphology is also maintained and the antigens are readily accessible for a wide variety of applications (IF, IHC etc.).
Sticking to scheduled time points when finishing and working up cell culture experiments
Special protocol for adherent cultured cells:
Application of CellCover is simple:
Remove cell culture medium, apply CellCover and incubate for two minutes. Then proceed with your standard protocol.
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